The report describes a recombinant human bone morphogenetic protein-2 (rhBMP-2) in combination, and regeneration of bone collagen (C). Unilateral critical size defects (CSDS) in a radius of 32 bones mature New Zealand White rabbits. Rabbits were evenly divided into four treatments: absorption C (bone morphogenetic protein -2 / C), and untreated autologous transplant absorb the C (Helistat ®), 35 μg of rhBMP-2 combined with the Central Securities Depository Management Office. The two euthanasia period were 4 and 8 weeks. X-rays taken the day of surgery, every two weeks, and in the long term measured ray impermeable percentage. Data analysis showed that the percentage of a time-varying radiopacity increased bone morphogenetic protein -2 / C composite rhBMP-2 / C histological examination found eight weeks of treatment, new bone contour. Quantitative histomorphometry, the Correctional Services Department (CSD) and group C was significantly less than the new self-body bone or bone morphogenetic protein-2 / C (P ≤ 0.05) occurred. The results showed that the bone morphogenetic protein -2 / C may be an effective treatment to restore segmental bone defects