Protein and recombinant human bone morphogenetic protein-2 with polyglycolic acid porous microspheres and autologous blood clots combination to treat large defect in rabbit femoral shaft defect model of bone induction test. Two kinds to cm nonuniting defects is surgery created 50 male New Zealand white rabbits, in the bilateral ulna. Then implanted a paste polyglycolic acid / blood clots combination of the mixed recombinant human bone morphogenetic protein-2 with five different concentrations of each defect. Forearm radiology assessment of eight weeks, bi-weekly schedule. After 8 weeks, all the animals were sacrificed and forearm radiographed. X-rays, and then score by three independent observers rate of bone formation and healing. Burstein torque tester mechanical strength test, reversing the limbs of the United States. Nonunion limbs, histology, unity limbs each dose group. The imaging assessment shows Healing Ft defects having the higher the rate of bone formation was a dose-dependent response at the extremities of the limbs of the high-dose than the low dose. The Union has made 100% of the highest dose of limbs, while only 50% of the lowest dose, limb bone healing. Implanted carrier alone achieved union without defects. The biomechanics research shows that the age-matched control group was significantly more severe bone. The seemingly normal osteoid histological analysis showed that normal bone formation. These dose-response data further support the role of recombinant human bone morphogenetic protein-2 as a powerful form occurs in bone regeneration.