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17 octobre 2012

Guanine nucleotide exchange structure foundation run regulator ChromosomeCondensation (RCC1)

RCC1 (regulator chromosomecondensation), beta propeller chromatin-binding protein, guanine nucleotide exchange factor (GEF) for the nuclear GTP-binding protein contingent. Here, we report the 1.8 angstrom crystal structure Ran • RCC1 complex nucleotide fund the multi-step reaction in the middle of the case. In contrast to previous structures, the phosphate-binding region of the nucleotide binding sites are Perturbed only slight, it may be due to the presence of the polyvalent anion in the P ring. Biochemical experiments indicate that the stability of the sulfate ion Ran • RCC1 complex and inhibition of guanine nucleotide dissociation. Based on structural and biochemical evidence, we propose a unified under the Global Environment Facility through the interactive mechanism of P ring of acidic residues lysine overall reaction is a crucial factor.

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